Figure 1
From: DNA mismatch repair and TP53 defects are early events in uterine carcinosarcoma tumorigenesis
Representative examples of molecular and immunohistochemical findings for carcinosarcomas. (a) Concordant MSI and MLH1 IHC in case 1689. Left two panels show abnormal PCR products characteristic of MSI (arrows) for D17S250 and D2S123 markers in both the epithelial and mesenchymal components. Lack of immunodectable MLH1 protein in the serous carcinoma and chondrosarcoma components of this tumor is illustrated in the right two panels (positive staining in endothelial cells in the sarcomatous element serves as internal positive control). (b) Concordant LOH at D17S250 and rs12602312 (Mae II RFLP) in the carcinomatous and sarcomatous elements of case 1239. Arrowheads indicate alleles lost in the cancer (left two panels). p53 overexpression in the serous carcinoma and mixed (rhabdomyosarcoma and undifferentiated) sarcoma from case 1239 (right two panels). (c) Retention of heterozygosity in both the carcinomatous and sarcomatous elements of case 1848 (left panel). Allelic retention of 17p shown by Cac8I restriction digestion of a 17p SNP (rs1014263). IHC demonstrates p53 overexpression in the leiomyosarcoma but not in the endometrioid carcinoma component (right two panels). C: carcinoma; S: sarcoma; α-MLH1: MLH1 antibody; α-p53: p53 antibody.
