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How to find, identify and quantitate the sugars on proteins

A new sequenator allows the identification, quantification and characterization of sites of glycosylation on proteins, making it possible to analyse the glycosylation at serine and threonine sites in mucin-like domains.

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References

  1. Wrotnowski, C. Genet. Eng. News (15 May) 7–29 (1996).

  2. Grinnell, B. W., Walls, J. D. & Gerlitz, B. J. Biol. Chem. 266, 9778–9785 (1991).

    CAS  PubMed  Google Scholar 

  3. Shakin-Eshleman, S. H., Spitalnik, S. L. & Lakshmi, K. J. Biol. Chem. 271, 6363–6366 (1996).

    Article  CAS  PubMed  Google Scholar 

  4. Jenkins, N., Parekh, R. B. & James, D. C. Nature Biotechnol. 14, 975–981 (1996).

    Article  CAS  Google Scholar 

  5. Harris, R. J. & Spellman, M. W. Glycobiology 3, 219–224 (1993).

    Article  CAS  PubMed  Google Scholar 

  6. Gooley, A. A., Classon, B. J., Marschalek, R. & Williams, K. L. Biochem. Biophys. Res. Comm. 178, 1194–1201 (1991).

    Article  CAS  PubMed  Google Scholar 

  7. Gooley, A. A., Marschalek, R. & Williams, K. L. Genetics 130, 749–756 (1992).

    CAS  PubMed  PubMed Central  Google Scholar 

  8. Pisano, A., Redmond, R. W., Williams, K. L. & Gooley, A. A. Glycobiology 3, 429–435 (1993).

    Article  CAS  PubMed  Google Scholar 

  9. Pisano, A., Packer, N. H., Redmond, J. W., Williams, K. L. & Gooley, A. A. Glycobiology 4, 837–844 (1994).

    Article  CAS  PubMed  Google Scholar 

  10. Zachara, N. E. et al. Eur. J. Biochem. 238, 511–518 (1996).

    Article  CAS  PubMed  Google Scholar 

  11. Pisano, A., Packer, N. H., Redmond, J. W., Williams, K. L. & Gooley, A. A. in Methods in Protein Structural Analysis (eds Atassi, M.Z. & Appella, E.) 69–80 (Plenum, New York, 1995).

    Book  Google Scholar 

  12. Pisano, A., et al. in Techniques in Glycobiology (eds Townsend, R. & Hotchkiss, A.) 299–320 (Marcel Dekker, New York, 1996).

    Google Scholar 

  13. Redmond, J. W. et al. Glycoconjugate Analysis Patent: PCT/AV 94/00164.

  14. Carr, S. A., Hemling, M. E., Bean, M. F. & Roberts, G. D. Analyt. Biochem. 63, 2802–2824 (1991).

    Article  CAS  Google Scholar 

  15. Carr, S. A., Huddleston, M. J. & Bean, M. F. Protein. Sci. 2, 183–196 (1993).

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  16. Gooley, A. A. et al. in Techniques in Protein Chemistry VI (ed. Crabb, J.W.) 83–90 (Academic, New York, 1995).

    Book  Google Scholar 

  17. Bures, E. J. et al. in Methods in Protein Structural Analysis (eds Atassi, M. Z. & Appella, E.) 57–68 (Plenum, New York, 1995).

    Book  Google Scholar 

Download references

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Author notes

  1. (keith.williams@mq.edu.au) are members of the Macquarie University Centre for Analytical Biotechnology which supports the Australian Proteome Analysis Facility at Macquarie University, Sydney, New South Wales 2109, Australia. The authors acknowledge support for their glycobiology research from the Australian Research Council the National Health & Medical Research Council, NovoNordisk (Denmark), Beckman Instruments (California) and Beckman Australia. Special thanks to Phill Isaacs. For more information fill in reader enquiry number 100

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    Gooley, A., Williams, K. How to find, identify and quantitate the sugars on proteins. Nature 385, 557–559 (1997). https://doi.org/10.1038/385557a0

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