FIGURE 1 | Modern Pathology

FIGURE 1

From: Laser Capture Microdissection–Guided Fluorescence In Situ Hybridization and Flow Cytometric Cell Cycle Analysis of Purified Nuclei from Paraffin Sections

FIGURE 1

Laser capture microdissection/fluorescence in situ hybridization of normal breast epithelial cells. Twenty-micron paraffin sections of formalin-fixed normal breast tissue were subjected to laser capture of normal epithelial cells. The nuclei were prepared and subjected to fluorescence in situ hybridization using CCND1 (red, large arrows) and RB1 (green, small arrows) cosmid genomic probes. Nuclei show the expected normal copy number for each gene of two to four, depending on the stage of cell cycle of the cells.

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