FIGURE 2 | Modern Pathology

FIGURE 2

From: Laser Capture Microdissection–Guided Fluorescence In Situ Hybridization and Flow Cytometric Cell Cycle Analysis of Purified Nuclei from Paraffin Sections

FIGURE 2

Laser capture microdissection/fluorescence in situ hybridization (FISH) of infiltrating duct carcinoma cells. Twenty-micron paraffin sections from two different cases of infiltrating duct carcinoma were subjected to laser capture of cells or cell groups from formalin-fixed breast cancer tissue (A–C, D–F). The nuclei were prepared and subjected to FISH using CCND1 (red, large arrows) and RB1 (green, small arrows) cosmid genomic probes and compared with FISH of touch preparations of the fresh tumors. A and D, typical histology of the two cases, respectively. B and E, FISH of the touch preparations. Amplification of CCND1, illustrated by an increase of gene copy number greater than six to eight, are shown in both cases. C and F, FISH of the laser capture microdissection preparations. In all cases, RB1 copy number is normal.

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