FIGURE 3 | Modern Pathology

FIGURE 3

From: Laser Capture Microdissection–Guided Fluorescence In Situ Hybridization and Flow Cytometric Cell Cycle Analysis of Purified Nuclei from Paraffin Sections

FIGURE 3

Laser capture microdissection (LCM)/flow cytometry of infiltrating duct carcinoma cells. Twenty-micron paraffin sections from the two cases shown in Figure 2 were subjected to LCM and nucleus isolation as previously described. Nuclei were stained with propidium iodide and analyzed as described in the “Methods” section. A, the analysis of the cells shown in Figure 2A–C, with 92.8% G1, 7.2% S, and no significant G2 phase populations of nuclei. In this specimen, approximately 2000 nuclei were available for analysis from one LCM cap. B, the analysis of the cells shown in Figure 2D–F, with 76.3% G1, 22.3% S, and 1.4% G2 phase. In this specimen, approximately 5000 nuclei were available for analysis from one LCM cap. Neither specimen shows significant aneuploidy.

Back to article page