TABLE 1 Antibodies Used in Study of Pulmonary Amyloidomas and Lymphomas with Amyloid
Antigen | Source | Dilution | Antigen Retrieval Method | Specificity |
|---|---|---|---|---|
CD3 | Dako, Carpinteria, CA | 1:400 | Microwave in citrate buffera | T cell |
CD5 | Novocastra, Burlingame, CA | 1:80 | Microwave in Tris-EDTA | T cell |
CD10 | Novocastra | 1:15 | Microwave in citrate | Precursor and germinal center B cell |
CD20 | Dako | 1:60 | Microwave in citrate | B cell |
CD21 | Dako | 1:30 | Ventana protease 1 for 12 minb | Mantle and marginal zone B cell, FDC |
CD23 | The Binding Site, San Diego, CA | 1:200 | None | Mantle and marginal zone B cell, FDC |
CD43 | Dako | 1:400 | Microwave in citrate + Ventana protease | T cells, some B cells, myeloid |
CD79a | Dako | 1:50 | Microwave in citrate | B cells |
Kappa light chain | Neomarkers, Fremont, CA | 1:100 | Microwave in Tris-EDTA | B-cell subset |
Lambda light chain | Neomarkers | 1:100 | Microwave in Tris-EDTA | B-cell subset |
IgA | Dako | 1:600 | Microwave in citrate | B-cell subset |
IgD | Dako | 1:300 | Microwave in citrate | B-cell subset |
IgG | Dako | 1:2000 | Microwave in citrate | B-cell subset |
IgM | Dako | 1:4000 | Microwave in citrate | B-cell subset |
Cyclin D1 | Santa Cruz Biotech, Santa Cruz, CA | 1:100 | Microwave in Tris-EDTA | Mantle cell lymphoma |
