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Reproducibility: Respect your cells!

Numerous variables can torpedo attempts to replicate cell experiments, from the batch of serum to the shape of growth plates. But there are ways to ensure reliability.

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References

  1. Khodabukus, A. & Baar, K. J. Cell. Biochem. 115, 2198–2207 (2014).

    Article  CAS  Google Scholar 

  2. Hughes, P., Marshall, D., Reid, Y., Parkes, H. & Gelber, C. Biotechniques 43, 575–586 (2007).

    Article  CAS  Google Scholar 

  3. Sikora, M. J., Johnson, M. D., Lee, A. V. & Oesterreich, S. Endocrinology http://dx.doi.org/10.1210/en.2016-1297 (2016).

  4. Olarerin–George, A. O. & Hogenesch, J. B. Nucleic Acids Res. 43, 2535–2542 (2015).

    Article  Google Scholar 

  5. Versteegen, R., Plavsic, M., Nims, R., Klostermann, R. & Hemmerich, K. BioProcess. J. 15, 5–11 (2016).

    Article  Google Scholar 

  6. Kim, H. J. & Ingber, D. E. Integr. Biol. 5, 1130–1140 (2013).

    Article  CAS  Google Scholar 

  7. Seo, D. W., Choi, H.-S., Lee, S. P. & Kuver, R. Am. J. Physiol. Gastrointest. Liver Physiol. 287, G1247–G1256 (2004).

    Article  CAS  Google Scholar 

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Baker, M. Reproducibility: Respect your cells!. Nature 537, 433–435 (2016). https://doi.org/10.1038/537433a

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