Figure 5
From: Establishment and characterisation of six human biliary tract cancer cell lines
Hypermethylation of E-cadherin gene in SNU-biliary tract cancer cell lines. (A) RT–PCR analysis of E-cadherin gene. Lane numbers (1–9) indicate cell lines: SNU-245, SNU-308, SNU-478, SNU-869, SNU-1079, SNU-1196, SNU-1 (gastric carcinoma cell line, positive control for methylation of E-cadherin gene, A-431(negative control), and water only. β-actin is RT–PCR control for mRNA expression. (B) RT–PCR analysis after 5-aza-2′-deoxycytidine treatment. It is note that E-cadherin gene is re-expressed after 5-aza-2′-deoxycytidine treatment. β-actin is RT–PCR control for mRNA expression. (C) Methylation specific PCR analysis after sodium bisulphite modification. It is evident that SNU-478 and SNU-1079 lines are methylated in CpG island of promoter region in the E-cadherin gene.
