Figure 5
From: L523S, an RNA-binding protein as a potential therapeutic target for lung cancer
Western blot analysis of immunoreactivity of lung cancer patient antibody to L523S. (A) Silver staining of recombinant L523S protein purified through nickel chromatography (lane 2). The arrow in (A) points to L523S protein confirmed by N-terminal sequencing analysis. (B) L523S recombinant protein was probed with lung pleural effusion from patient #3 (lane 1′), #12 (lane 2′), and #15 (lane 3′). Notice that stronger antibody titre in #12 detected by ELISA analysis (Table 2) also has increased immunoreactivity to L523S protein by Western blot analysis.
