Table 1 Flow cytometric analysis of ovalbumin expression and immune cell infiltration in EL-4 , progressive E.G7-OVA and regressive E.G7-OVA tumours

From: Early detection of tumour immune-rejection using magnetic resonance imaging

 

Tumour type

Antigen

EL-4

E.G7-OVA (regressive)

E.G7-OVA (progressive)

Ovalbumin

3.4±0.2

1608±480a

309±66

CD8

150±23

272±27a

174±19

CD68

64±17

177±35b

99±29

  1. At 13 days after implantation, single-cell suspensions were prepared by disaggregating solid tumour material. These cell suspensions were then incubated with the relevant primary antibody and fluorochrome-conjugated secondary antibody, as described in the Materials and Methods section. Three tumours were used in each group and the numbers represent the mean±s.e.m. of the fluorescence intensities in arbitrary units.
  2. aStatistically significantly different from EL-4 and progressive E.G7-OVA tumours, P<0.01;
  3. statistically significantly different from EL-4 tumours, P<0.01.
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