Table 1 Primer sequences for PCR–SSCP analysis of the ST7gene
Primer sequences (5′–3′) | ||||
|---|---|---|---|---|
Exon | Product size (bp) | Tm (°C) | Sense | Antisense |
1a | 273 | 62 | gaatcatcccggcagacac | gcgcgagttgcactaacttt |
1b | 234 | 58 | agcagagaggagcgctgaa | ttgcactaactttccggggc |
2 | 148 | 62 | ccttgttcttctccctttctc | ttaaatgagaaggactccacc |
3 | 230 | 58 | aacagtgaccataaacacgct | aaataatattgcaaactgaagg |
4 | 162 | 62 | gtagtgtcactgaacttacgc | ctgtctttgctctctgaacc |
5 | 369 | 58 | aggtcttgcttttctctctca | gaggggactcatttcaacata |
6 | 220 | 62 | ggattgacttggtgttttctc | atcctccagttcaaatgcagt |
7 | 176 | 62 | gtgactctctctgaatgttcc | tcatttggttagaagtagggc |
8 | 237 | 62 | ggctttgtaattgatggtggc | acaattctgatccccccaatgc |
9 | 342 | 58 | tcaacatcctcactcaaaagc | tctgtaagccactgatcccaa |
10 | 195 | 58 | attccttggtttcttctgccc | gggaaaatacatcaaaagagg |
11 | 191 | 58 | cctgcaaacttatgtgttcct | aacacatctcaattccggtca |
12 | 168 | 62 | ggatggtttttgtctttctgc | atcataacgagttcctgtggg |
13 | 237 | 62 | attaacacaagtgtgtcctgc | ttagcaccttttcatgctctt |
14 | 209 | 62 | cacaaacattggacatctctg | ctggctgaagagaggtgaga |
15 | 351 | 58 | gggtcagatgttggctatgg | cttggctttccccatccatt |
16a | 200 | 62 | ggtttctgctgacttctgtg | aaggagttggcacagaggag |
16b | 225 | 58 | aggcgagtgcaatcagaaag | gaggaggagcagttttggtg |
