Figure 1

BRAF gene mutations in NHLs. SSCP (A–C) and DNA sequencing analyses (D–F) of DNA from tumours (lane T) and normal tissues (lane N). Exon 11 (A, B) and exon 15 (C) of BRAF were amplified. SSCPs of DNA from the tumours show wild-type bands and additional aberrant bands (arrows) as compared to SSCP from normal cells from the same patients. (D) Sequencing analysis from the aberrant band in (A). There is a G to C transversion at nucleotide 1403 of BRAF (arrow) in tumour tissue as compared to normal tissue. (E) Sequencing analysis from the aberrant band in (B). There is a G to C transversion at nucleotide 1402 of BRAF (arrow) in tumour tissue as compared to normal tissue. (F) Sequencing analysis from the aberrant band in (C). There is an A to G transition at nucleotide 1778 of BRAF (arrow) in tumour tissue as compared to normal tissue. Numbering of cDNA of BRAF was made in respect to the ATG start codon (GenBank).