Figure 4
Immunohistochemical analysis of gastric tumours after a single dose of ZD6126. Representative images of tumour sections stained with haematoxylin and eosin (column 1; × 40 magnification), stained for endothelial cell proliferation by immunofluorescence for CD31/BrdU (column 2; × 100) and stained for endothelial cell apoptosis by immunofluorescence against CD31/TUNEL (column 4, × 100). A single dose of ZD6126 caused a significant decrease in the number of dividing endothelial cells (as determined by colocalisation) and an increase in endothelial cells undergoing apoptosis, such that microvessel density was decreased by day 5.
