Figure 3
Extracellular metabolites of CB 1954 in SiHa-NTRpuro single-cell suspensions (A, B) and multicellular layers (C, D). (A) HPLC chromatogram (absorbance) of extracellular medium after incubation of cells with 100 μ M CB 1954 for 3 h at 2.5 × 105 cells ml−1. U1, unknown product; *phenol red. (B) Time course of formation of extracellular metabolites as in (A). (C, D) Diffusion of CB 1954 metabolites out of SiHa-NTRpuro MCLs during exposure to CB 1954 (initial concentration 100 μ M in the donor compartment). Symbols as in (B).
