Figure 6
Extracellular metabolites of SN 23862 in SiHa-NTRpuro single-cell suspensions (A, B) and multicellular layers (C, D). (A) HPLC chromatogram of extracellular medium after incubation of cells with 100 μ M SN 23862 for 3 h at 2.5 × 105 cells ml−1. U2, unknown product; *phenol red. (B) Time course of formation of extracellular metabolites as in (A). (C, D) Appearance of metabolites in medium during incubation of SiHa-NTRpuro MCLs to SN 23862 (initial concentration 100 μ M in the donor compartment). Symbols as in (B).
