Table 1 Kinetics of metabolic consumption of dinitrobenzamide prodrugs by NTR-expressing and control cells at 106 cells ml−1, determined by monitoring loss with time in the extracellular medium

From: 2-Amino metabolites are key mediators of CB 1954 and SN 23862 bystander effects in nitroreductase GDEPT

 

First-order rate constant k (h−1)a

Cell line

CB 1954

SN 23862

V79puro

0.0010±0.0004

0.0020±0.0001

V79-NTRpuro

0.08±0.03

0.17±0.003

SiHa

0.0066±0.0001

<0.001

SiHa-NTRpuro

0.22±0.06

0.6±0.2

  1. aExperiments were performed using a cell density of 5 × 106 ml−1 for the V79 lines and SiHa, and 2.5 × 105 ml−1 for SiHa-NTRpuro. Values are means±s.e.m. for 2–3 cell suspensions, corrected to a cell density of 106 ml−1.
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