Table 1 Primer sequences

From: Activity (transcription) of the genes for MLH1, MSH2 and p53 in sporadic colorectal tumours with micro-satellite instability

Gene

Region

Primer sequence

Direction

Size (bp)

MLH1

5′

-TGTTAAAGAGGGAGGCCTGA-

Sense

92

  

-TGAACCTTTCACATACAATATCCA-

Antisense

 
 

3′

-AAGAAGGCTGAGATGCTTGC-

Sense

102

  

-CAAAGGGGGCACATAGTTGT-

Antisense

 

MSH2

5′

-AAGGCATCCAAGGAGAATGA-

Sense

106

  

-TGGAAGCTGACATATCATTGTTAC-

Antisense

 
 

3′

-GAGTCTCCACGTTCATTGGCT-

Sense

103

  

-GAAGTTCCTCTTCCCAATTCA-

Antisense

 

p53

5′

-CCCCTCTGAGTCAGGAAACA-

Sense

93

  

-AATCATCCATTGCTTGGGAC-

Antisense

 
 

3′

-AGGCCTTGGAACTCAAGGAT-

Sense

106

  

-TTATGGCGGGAGGTAGACTG-

Antisense

 

GAP-3

—

-GAGTCAACGGATTTGGTCGT-

Sense

103

  

-TTGAGGTCAATGAAGGGGTC-

Antisense

 
  1. Primer sequences used for RT–PCR analyses.
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