Figure 3

Effect of 15d-PGJ₂ on EGFR-mediated Stat3 signalling. (A) Representative oral SCC cells (SCC9 and SCC25) were treated with NM, 15d-PGJ₂ (20 μ M) for 45 min, or stimulated with TGF-α (25 ng ml⁻¹) for 45 min in the presence or absence of 1 h pretreatment with 15d-PGJ₂ PG (20 μ M). Cells were blotted with phospho-Stat3 antibody (Y705) and subsequently stripped and reprobed with Stat3 antibody. (B) Representative oral SCC cells (SCC9 and SCC25) were treated with NM or 15d-PGJ₂ PG (20 μ M) for 10 min. EGFR constitutive phosphorylation was determined by immunoprecipitation with EGFR antibody and Western blotting for phosphorylated (PY) and total EGFR.