Table 3 Conditions of sequence-specific PCR cycling reactions

From: Wild-type oestrogen receptor beta (ERβ1) mRNA and protein expression in Tamoxifen-treated post-menopausal breast cancers

  

Condition

Oligonucleotide

Cycle

Denaturation phase

Annealing phase

Extension phase

ERα and β-actin

35

94°C, 15 s

58°C, 15 s

72°C, 30 s

ERβ1

40

94°C, 30 s

64°C, 40 s

72°C, 45 s

HPRT

36

94°C, 30 s

60°C, 60 s

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