Figure 3
Aspirin induces IκBα degradation and nuclear translocation of p65 in colorectal cancer (CRC) lines. Following aspirin treatment, cytoplasmic extracts were made from untreated and treated cells and probed with sheep polyclonal IκBα antibody. Western blot analysis shows that aspirin treatment (0–10 mM) for 16 h induces IκBα degradation in a concentration-dependent manner in the HCT-116, HCT116+ch3 and HCT-116p53−/− CRC cell lines (A). The Western blot shown is representative of at least three independent experiments and Cu/Zn SOD was used as a control for protein loading. Micrographs (63 ×) of immunocytochemically stained cells show that aspirin treatment (10 mM) for 16 h induces nuclear accumulation of p65 in the HCT-116, HCT116+ch3 and HCT-116p53−/− CRC cell lines (B).
