Figure 4

Aspirin induces repression of NFκB-driven transcription in colorectal cancer (CRC) lines. CRC cells were transfected with the ConA NFκB dependent luciferase reporter construct, containing three κB binding sites, or the equivalent plasmid with κB consensus sites deleted (ConA ΔκB). All cells were cotransfected with the control CMV-β-galactosidase plasmid. Following 16 h treatment with 0–10 mM aspirin, luciferase and β-galactosidase assays were performed on cell lysates and relative luciferase activity calculated. The graphs represent three independent experiments and the bars on the graphs are standard error bars.