Figure 7
From: Atiprimod blocks STAT3 phosphorylation and induces apoptosis in multiple myeloma cells
Atiprimod inhibits primary MM cell proliferation. (A) Low-density BM cells from three patients with MM and incubated in the presence of Atiprimod at concentrations ranging from 1 to 8 μ M and analysed using the MTT assay. (B) The effect of Atiprimod on MM colony-forming cells was studied on marrow cells from five patients with MM. After fractionation, myeloma cells were cultured in the presence of Atiprimod at concentrations ranging from 1 to 8 μ M. Multiple myeloma colonies are presented as percentages of control (the mean number of colonies obtained from patients 1 through 5 in the absence of Atiprimod was 82, 243, 133, 179, and 467, respectively). (C) The top panel depicts two typical MM colonies. Of note are the large cells with myeloma cell morphology at the periphery of the colony. The middle panel shows the products of PCR amplification of DNA from the original marrow of patient 2 and from seven single colonies microaspirated at random using VH3–JH consensus primers. The myeloid leukaemia cell line OCIM2 was used as a negative control (Neg. Cont.). Actin was used as an amplification control (MW denotes molecular weight). The lower panel shows the nucleotide sequences of amplified products of DNA from the D region of the diagnostic marrow (1) and of three different colonies (2–4). The middle and lower panels demonstrate that the MM colonies originated from the neoplastic clone.
