Figure 3 | British Journal of Cancer

Figure 3

From: In vitro evaluation of cancer-specific NF-κB-CEA enhancer–promoter system for 5-fluorouracil prodrug gene therapy in colon cancer cell lines

Figure 3

The transcriptional activity and specificity of CEA205 and CEA421 basal promoters in combination with or without κB4 in CRC and normal human cell cultures. (A) The cells were co-transfected with luciferase expression vectors (pCEA205-Luc, pκB4-CEA205-Luc, pCEA421-Luc, pκB4-CEA421-Luc and pGL3-Basic) and Renilla expression vector (pRL3-SV40). The luciferase activity of each transfection was normalised by the Renilla reading. The luciferase activity is represented by the ratio of the specific promoter over the activity of pGL3-Basic. The column represents the mean of three measurements and the bar represents the s.d. (B and C) The cells were singly transfected with pGL3-SV40- or pκB4-CEA205-Luc and the absolute luciferase activity in equal amount of protein from different transfections was compared.

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