Figure 4

The TP enzyme status and cytotoxicity of 5′-DFUR in transiently transfected CRC cell lines. (A) Thymidine phosphorylase protein expression levels determined by Western blot. Vinculin: protein loading control. (B) Thymidine phosphorylase enzyme activity in different transfected cells. (C and D) The inhibitory effect of 5′-DFUR on the transiently transfected RKO (C) and H630 (D) cells. The curves were derived from three replicates and bars represent s.d.