Figure 1

Protein levels of iNOS, eNOS, and nNOS in cisplatin-sensitive OV2008 and in cisplatin-resistant C13^* cells were determined using western blot analysis. (A) Basal iNOS levels were significantly higher in OV2008 cells compared with C13^* cells. Cisplatin (CDDP, 10 μ M, 24 h) significantly upregulated iNOS in OV2008, but not in C13^* cells. (B and C) Basal levels of eNOS and nNOS were significantly lower in OV2008 cells compared with C13^* cells. Cisplatin (5 and 10 μ M, 24 h) significantly downregulated both eNOS and nNOS in OV2008, but not in C13^*, cells. The bar graphs show mean±s.e.m. of protein levels of five independent experiments. ^*P<0.05, compared with control (no cisplatin). ^**P<0.01, compared with control (no cisplatin). ^#P<0.05, comparing basal levels in control OV2008 and in C13^* cells. ^##P<0.01, comparing basal levels in control OV2008 and in C13^* cells.