Figure 4
From: Three dysregulated miRNAs control kallikrein 10 expression and cell proliferation in ovarian cancer

Effect of transfection of miR-224 and miR-516a on luciferase activity as measured with the pMIR–KLK10. Cells were co-transfected with the empty vector, pMIR and hsa-miR-224 precursor molecule, the empty vector, pMIR and hsa-miR-516a precursor molecule, the cloned vector, pMIR–KLK10 and hsa-miR-224 precursor molecule, pMIR–KLK10 and hsa-miR-516a precursor molecule, or pMIR–KLK10 only. There was a decrease in luciferase activity when cells were co-transfected with pMIR–KLK10 and either miR-224 or miR-516a.