Figure 4 | British Journal of Cancer

Figure 4

From: Basic fibroblast growth factor in the bone microenvironment enhances cell motility and invasion of Ewing's sarcoma family of tumours by activating the FGFR1–PI3K–Rac1 pathway

Figure 4

Bone marrow stromal cells (BMSCs) expressed bFGF and conditioned medium from BMSC (CM/BMSC) induced the motility of ESFT cells by stimulating bFGF/FGFR1 signalling. (A) bFGF expression in BMSC was assessed by real-time PCR. IL-1β-treated human umbilical vein endothelial cells (HUVECs) were used as a positive control. (B) CM/BMSC induced the tyrosine phosphorylation of FGFR1 in RD-ES cells. CM-BMSC was harvested from BMSCs that were cultured in a 24-well plate for 72 h. Treating RD-ES cells with CM/BMSC resulted in the tyrosine phosphorylation of FGFR1, and this phosphorylation was reduced by 2 h pre-treating with an anti-bFGF neutralising antibody (5 or 20 μg ml−1) to the CM/BMSC. Actin was used as a loading control. Data are representative of at least three independent trials. (C) The chemotaxis of RD-ES cells in response to CM/BMSC was assessed by the chemotaxis assay. RD-ES cells (2 × 105) were plated onto the upper chamber. CM/BMSC enhanced the chemotaxis of RD-ES cells, whereas an anti-bFGF-neutralizing antibody (5 μg ml−1) and SU5402 (20 μ M) impaired the chemotaxis of RD-ES cells towards CM/BMSC. Data are depicted as mean±s.d. of at least three independent experiments. ***P<0.001.

Back to article page