Figure 5
Effects of pharmacological inactivation of the PI3K, MAPK, and PLC-γ pathways on bFGF-induced chemotaxis of ESFT cells. LY294002 (PI3K inhibitor), PD98059 (MAPK inhibitor), and U-73122 (PLC-γ inhibitor) were used. The effects of each inhibitor on bFGF-induced chemotaxis of RD-ES cells were assessed using a chemotaxis assay with bFGF (10 ng ml−1). LY294002 significantly inhibited bFGF-induced chemotaxis, whereas PD98059 and U-73122 had no effect. In all, 2 × 105 cells were plated onto the upper chamber. bFGF and each inhibitor were added at the same time. Experiments were performed in triplicate and repeated at least three times. Data are shown as mean±s.d. **P<0.01 vs bFGF with DMSO, #, not significant.
