Figure 1

Prevalence of Th17 and Th1/Th17 cells in peripheral blood of HNSCC patients. The CD4+ T cells were isolated from peripheral blood of HNSCC patients and healthy controls and subsequently incubated for 4 h in the presence of PMA/Ionomycin and Brefeldin A. The cells were stained with CD3-FITC, CD4-PerCP, IL-17-APC, ROR2C-PE and IFNγ-PECy7 and analysed by flow cytometry. The CD3 and CD4+ T cells were gated in the FACS analysis. (A) Comparison of the prevalence of Th17 cells in peripheral blood of healthy persons (n=25) and HNSCC patients (n=25) with P<0.05. The data is expressed as the frequency of Th17 cells in the lymphocyte population. The box plots show the median (middle line), 25th and 75th percentiles (box), the extreme values (whiskers, which indicate the 90th and 10th percentile) and the outliers (circles). (B) Prevalence of Th17/Th1 cells in peripheral blood of head and neck cancer patients (n=25) compared with healthy controls (n=25) with P<0.05. The data is expressed as the frequency of Th17/Th1 cells in the Th17 population.