Figure 5
The proliferation of HNSCC cells is reduced in the presence of Th17 cells. (A) PCI-13 cells were incubated with Th17 cells, Th1 cells and DMEM in a transwell chamber over 96 h and the cell number was checked every 24 h with MTT reagents. The lines are the mean of eight-fold repeats±s.d. (B) The HNSCC cell line PCI-13 were incubated with Th17 cells, Th17-depleted PBMCs and DMEM, and after 24 h of incubation, the PCI-13 cells were harvested and stained with Annexin and PI and analysed by flow cytometry. The HNSCC cells incubated with Th17 cells show a significant upregulation of Annexin V as well as PI (n=15). The bars show the mean of the positive cells±s.d. with P<0.05. (C) Expression of IL-17R (first bar), IL-21R (second bar) and IL-22R (third bar) on tumour cells analysed by flow cytometry. The bars show the mean of eight-fold repeats±s.d. (D) PCI-13 cells were incubated with Th17 cells, Th17 cell supernatant and DMEM over 96 h and the tumour cell number was checked every 24 h with MTT reagents. The lines are the mean of eight-fold repeats±s.d.
