Figure 5

miR-22 targets human HDAC4. (A) Sequence alignment of miR-22 and its conserved target site in HDAC4 3′ UTR (downloaded from TargetScan). (B) Luciferase expression of the indicated construct co-transfected with control RNA or miR-22 mimics. Data are shown as mean±s.d. (n=6). ^**P<0.01. (C) Hep3B and SMMC7721 cells were transfected as in Figure 4A. After 48 h, human HDAC4 and β-actin were detected by western blot.