Abstract
Transrectal ultrasound (TRUS) guided prostate biopsy is standard diagnostic procedure for prostate cancer (PCa). However, possibility of dissemination of cancer cells by biopsy is not negligible. To investigate this possibility, we examined prostate specific antigen (PSA)-bearing cells in peripheral blood of the 108 patients before and after prostate biopsy. Peripheral blood samples were obtained from 108 patients with elevated serum PSA (sPSA) levels, who had undergone sextant prostate biopsy using TRUS. The presence of PSA-mRNA bearing cells was examined using the nested RT-PCR method enabling detection of one LNCaP cell diluted in 1 ml of whole blood. Among 108 patients, 62 and 46 were diagnosed with benign prostatic hyperplasia (BPH) and PCa, respectively. PSA-mRNA was detected in 3 PCa cases but in no BPH patients before and after biopsy, and in 16 BPH (25.8%) and in 21 PCa (45.7%) patients only after biopsy (P < 0.01). The patients with positive mRNA before biopsy had higher sPSA (P < 0.001), and those after biopsy had higher sPSA and PSA density (PSAD) levels (P < 0.05). Positive PSA-mRNA cases had more cancer involved biopsy cores than the negative PSA-mRNA cases (P < 0.001). Although further investigations are needed, the present findings suggest that prostate biopsy might scatter prostate cells in the blood stream especially in cases with high sPSA and, thus, might contribute to tumour spreading in the cases of prostate cancer. © 2001 Cancer Research Campaign http://www.bjcancer.com
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References
Berteau P, Gala JL, Eschwege P, Dumas F and Loric S (1999) The value of a reverse transcriptase polymerase chain reaction assay in preoperative staging and followup of patients with prostate cancer. J Urol 161: 924–926
Catalona WJ, Richie JP, Ahmann FR, Hudson MA, Scardino PT, Flanigan RC, deKernion JB, Ratliff TL, Kavoussi LR and Dalkin BL (1994) Comparison of digital rectal examination and serum prostate specific antigen in the early detection of prostate cancer: results of a multicenter clinical trial of 6,630 men. J Urol 151: 1283–1290
Corey E and Corey MJ (1998) Detection of disseminated prostate cells by reverse transcription-polymerase chain reaction (RT-PCR): technical and clinical aspects. Int J Cancer 77: 655–673
Corey E, Arfman EW, Oswin MM, Melchior SW, Tindall DJ, Young CY, Ellis WJ and Vessella RL (1998) Detection of circulating prostate cells by reverse transcriptase-polymerase chain reaction of human glandular kallikrein (hK2) and prostate-specific antigen (PSA) messages. Int J Cancer 77: 655–673
Crawford ED and DeAntoni EP (1993) PSA as a screening test for prostate cancer. Urol Clin North Am 20: 637–646
Crawford ED, Bennett CL, Stone NN, Knight SJ, DeAntoni E, Sharp L, Garnick MB and Porterfield HA (1997) Comparison of perspectives on prostate cancer: analyses of survey data. Urology 50: 366–372
de la Taille A, Olsson CA, Buttyan R, Benson MC, Bagiella E, Cao Y, Burchardt M, Chopin DK and Katz AE (1999) Blood-based reverse transcriptase polymerase chain reaction assays for prostatic specific antigen: long term follow-up confirms the potential utility of this assay in identifying patients more likely to have biochemical recurrence (rising PSA) following radical prostatectomy. Int J Cancer 84: 360–364
de la Taille A, Katz A, Cao Y, McKiernan J, Buttyan R, Burchardt M, Burchardt T, Hayek O, Olsson CA, Chopin DK and Sawczuk IS (2000) Blood-based RT-PCR assays of MN/CA9 or PSMA: clinical application in renal cancer patients. Urology 56: 393–398
Eskola JU, Hamalainen M, Nanto V, Rajamaki A, Dahlen P, Iitia A and Siitari H (1994) Detection of Philadelphia chromosome using PCR and europium-labeled DNA probes. Clin Biochem 27: 373–379
Fadlon EJ and Hamdy FC (1998) The use of flow cytometry and RT-PCR in the detection of circulating PSA-positive cells in prostate cancer. Method Mol Biol 92: 215–225
Fadlon EJ, Rees RC, McIntyre C, Sharrard RM, Lawry J and Hamdy FC (1996) Detection of circulating prostate-specific antigen-positive cells in patients with prostate cancer by flow cytometry and reverse transcription polymerase chain reaction. Br J Cancer 74: 400–405
Ferrari AC, Stone NN, Eyler JN, Gao M, Mandeli J, Unger P, Gallagher RE and Stock R (1997) Prospective analysis of prostate-specific markers in pelvic lymph nodes of patients with high-risk prostate cancer. J Natl Cancer Inst 89: 1498–1504
Fidler IJ (1990) Critical factors in the biology of human cancer metastasis: twenty-eighth G.H.A. Clowes memorial award lecture. Cancer Res 50: 6130–6138
Gomella LG, Raj GV and Moreno JG (1997) Reverse transcriptase polymerase chain reaction for prostate specific antigen in the management of prostate cancer. J Urol 158: 326–337
Greenlee RT, Murray T and Thun M (2001) Cancer statistics, 2001. Cancer J Clin 51: 15–36
Kiser GC, Totonchy M and Barry JM (1986) Needle tract seeding after percutaneous renal adenocarcinoma aspiration. J Urol 136: 1292–1293
Levine ES, Cisek VJ, Mulvihill MN and Cohen EL (1986) Role of transurethral resection in dissemination of cancer of prostate. Urology 28: 179–183
Liotta LA, Saidel MG and Kleinerman J (1976) The significance of hematogenous tumor cell clumps in the metastatic process. Cancer Res 36: 889–894
Mejean A, Vona G, Nalpas B, Damotte D, Brousse N, Chretien Y, Dufour B, Lacour B, Brechot C and Paterlini-Brechot P (2000) Detection of circulating prostate derived cells in patients with prostate adenocarcinoma is an independent risk factor for tumor recurrence. J Urol 163: 2022–2029
Moreno JG, Croce CM, Fischer R, Monne M, Vihko P, Mulholland SG and Gomella LG (1992) Detection of hematogenous micrometastasis in patients with prostate cancer. Cancer Res 52: 6110–6112
Moreno JG, O'Hara SM, Long JP, Veltri RW, Ning X, Alexander AA and Gomella LG (1997) Transrectal ultrasound-guided biopsy causes hematogenous dissemination of prostate cells as determined by RT-PCR. Urology 49: 515–520
Okegawa T, Noda H, Kato M, Miyata A, Nutahara K and Higashihara E (2000) Value of reverse transcription polymerase chain reaction assay in pathological stage T3N0 prostate cancer. Prostate 44: 210–218
Paik HC, Lee DY, Lee HK, Kim SJ and Lee KB (1994) Chest wall implantation of carcinoma after fine needle aspiration biopsy. Yonsei Medical Journal 35: 349–354
Raj GV, Moreno JG and Gomella LG (1998) Utilization of polymerase chain reaction technology in the detection of solid tumors. Cancer 82: 1419–1442
Sabbatini R, Federico M, Morselli M, Depenni R, Cagossi K, Luppi M, Torelli G and Silingardi V (2000) Detection of circulating tumor cells by reverse transcriptase polymerase chain reaction of maspin in patients with breast cancer undergoing conventional-dose chemotherapy. J Clin Oncol 18: 1914–1920
Saito T, Kimura M, Kawasaki T, Sato S and Tomita Y (1996) Correlation between integrin alpha 5 expression and the malignant phenotype of transitional cell carcinoma. Br J Cancer 73: 327–331
Smith EH (1984) The hazards of fine-needle aspiration biopsy. Ultrasound Med Biol 10: 629–634
Su SL, Boynton AL, Holmes EH, Elgamal AA and Murphy GP (2000) Detection of extraprostatic prostate cells utilizing reverse transcription-polymerase chain reaction. Semin Surg Oncol 18: 17–28
Voravud N, Shin DM, Dekmezian RH, Dimery I, Lee JS and Hong WK (1992) Implantation metastasis of carcinoma after percutaneous fine-needle aspiration biopsy. Chest 102: 313–315
Webber MM, Bello D, Kleinman HK, Wartinger DD, Williams DE and Rhim JS (1996) Prostate specific antigen and androgen receptor induction and characterization of an immortalized adult human prostatic epithelial cell line. Carcinogenesis 17: 1641–1646
Yamamoto S (1999) Cancer statistics digest. Comparison of crude and age-standarized death rates in Japan. Jpn J Clin Oncol 29: 649
Yuan JJ, Coplen DE, Petros JA, Figenshau RS, Ratliff TL, Smith DS and Catalona WJ (1992) Effects of rectal examination, prostatic massage, ultrasonography and needle biopsy on serum prostate specific antigen levels. J Urol 147: 810–814
Zisman A, Soffer Y, Siegel YI, Paz A and Lindner A (1997) Post ejaculation serum prostate-specific antigen level. Eur Urol 32: 54–57
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Hara, N., Kasahara, T., Kawasaki, T. et al. Frequency of PSA-mRNA-bearing cells in the peripheral blood of patients after prostate biopsy. Br J Cancer 85, 557–562 (2001). https://doi.org/10.1054/bjoc.2001.1924
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DOI: https://doi.org/10.1054/bjoc.2001.1924
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