Abstract
Purpose Oxidative mechanisms play a major role in the aetiology and pathogenesis of cataract, especially in age-related cataract. Our study aims to investigate systemic oxidant and antioxidant markers in cataract patients.
Methods The activity of erythrocyte catalase and the level of malondialdehyde in plasma were measured in 40 patients with cataract and 60 healthy control subjects. The malondialdehyde level, as an index of lipid peroxidation, was determined by thiobarbitüric acid reaction according to Yagi. The determination of catalase activity was measured by a method that was defined by Beutler. Catalase enzyme activity and malondialdehyde level were evaluated to find out whether there was a significant difference in these variables. Analysis of variance was used by forming a general linear model that takes age and gender as the covariate.
Results CAT activity was found to be 13 920.2±847.9 U/l in cataract patients and 16 061.3±1126.6 U/l in control subjects. CAT activity in cataract patients was significantly lower than the control subjects (P=0.008). Plasma MDA level is significantly higher in patients with cataract 4.47 ± 0.35 nmol/ml compared to the control subjects 2.94±0.26 nmol/ml (P=0.0001). There was no significant difference between different cataract subgroups when erythrocyte CAT activities and plasma MDA levels were compared (P=0.322, 0.062).
Conclusion This study shows that oxidant/antioxidant balances alter in the presence of cataract.
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References
Behnding A, Svensson B, Marklund SL, Karlsson K . Superoxide dismutase isoenzymes in the human eye. Invest Ophtalmol Vis Sci 1998; 39: 471–475.
Fecondo JV, Augusteyn RC . Superoxide dismutase, catalase and glutathione peroxidase in the human cataractous lens. Exp Eye Res 1983; 36: 15–23.
Spector A, Wang GM, Wang RR, Garner WH, Moll H . The prevention of cataract caused by oxidative stress in cultured rat lenses: I: H2O2 and photochemically induced cataract. Curr Eye Res 1993; 12: 163–179.
Pirie A . Photooxidation of proteins and comparison of photo-oxidised proteins with those of the cataractous human lens. Israel J Med Sci 1972; 8: 1567–1573.
Zigman S, Griess G, Yulo T, Schultz J . Ocular protein alterations by near UV light. Exp Eye Res 1973; 15: 255–264.
Alberti G, Oguni M, Podgor M, Sperduto RD, Tomarev S, Grassi C et al. Glutathione S-transferase M1 genotype and age-related cataracts. Invest Ophtalmol Vis Sci 1996; 37: 1167–1173.
Chylack Jr LT, Leske MC, McCarthy D, Khu P, Kashiwagi T, Sperduto R . Lens opacities classification system II (LOCS II). Arch Ophthalmol 1989; 107: 991–997.
Beutler E . Glutathion. In: Beutler E (ed) Red Cell Metabolism: A Manual Of Biochemical Methods. Grune and Stratton: New York 1975; pp 105–107.
Yagi K . Lipid peroxides and related radicals in clinical medicine. In: Armstrong D (ed) Free Radicals in Diagnostic Medicine. Plenum Press: New York 1994; pp 1–15.
Spector A . Oxidative stress-induced cataract: mechanisms of action. FASEB J 1995, 9: 1173–1182.
Gritz DC, Montes C, Atalla LR, Wu GS, Sevanian A, Rao NA . Histochemical localization of superoxide production in experimental autoimmune eveitis. Curr Eye Res 1991; 10: 927–931.
Niesman MR, Johnson KA, Penn JS . Therapeutic effect of liposomal superoxide dismutase in an animal model of retinopathy of prematurity. Neurochem Res 1997; 22: 597–605.
Alio JL, Ayala MJ, Mulet ME, Artola A, Ruiz JM, Bellot J . Antioxidant therapy in the treatment of experimental acute corneal inflammation. Ophthalmic Res 1995; 27: 136–143.
Alio JL, Artola A, Serra A, Ayala MJ, Mulet ME . Effect of topical antioxidant therapy on experimental infectious keratitis. Cornea 1995; 14: 175–179.
Hauet TH, Bauza G, Goujan M, Caritez C, Carretier M, Eugene M et al. Effect of trimetazidine on lipid preoccupation and phosphorus metabolites during cold storage and reperfusion of isolated perfused rat kidneys. Pharmacol Exp Ther 1998; 285: 1061–1067.
Jacques PF, Chylack LT, McGandy RB, Hartz SC . Antioxidant status in persons with and senile cataract. Arch Ophthalmol 1988; 106: 337–340.
Spector A . The search for a solution to senile cataract. Invest Ophthalmol Vis Sci 1984; 25: 130–146.
Babizhaev MA, Deev AI . Free radical oxidation of lipids and thiol groups in formation of a cataract. Biofizika 1986; 31: 109–114.
Kleiman NJ, Spector A . Quantitation of DNA damage in single human lens epithelial cells. Invest Ophthalmol Vis Sci 1992; 33: 79–99.
Feconda JV, Augesteyn RC . Superoxide dismutase, catalase and glutathione peroxidase in human cataractous lens. Exp Eye Res 1983; 36: 15–32.
Spector A, Garner WH . Hydrogen peroxide and human cataract. Exp Eye Res 1981; 33: 673–681.
Ohrloff C, Hockwin O . Superoxide dismutase (SOD) in normal and cataractous human lenses. Graefes Arch Clin Exp Ophthalmol 1984; 222: 79–81.
Orhan H, Marol S, Hepşen IF, Sahin G . Effects of some probable antioxidants on selenite-induced cataract formation and oxidative stress-related parameters in rats. Toxicology 1999; 139: 219–232.
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Ateş, N., Yildirim, Ö., Tamer, L. et al. Plasma catalase activity and malondialdehyde level in patients with cataract. Eye 18, 785–788 (2004). https://doi.org/10.1038/sj.eye.6700718
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DOI: https://doi.org/10.1038/sj.eye.6700718
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