Abstract
Aim of purpose
To observe the different expression of matrix metalloproteinases (MMPs) between pre- and postoperation of sham cataract surgery in vitrohuman lens capsule bag model from the same donor eye in order to investigate a possible role of MMPs in posterior capsule opacification (PCO).
Methods
Sham cataract surgeries were performed in six human donor eyes. Immunohistochemical staining was used to detect the expression of MMP-2 and -9 of human lens epithelial cells (LECs) on the anterior capsulorhexis. LEC migration on posterior capsule of human lens from the same donor eye was observed in a modified capsule bag model without pin. Total MMP-2 and -9 protein production were determined by enzyme-linked immunosorbent assay at days 2, 10, 20, and 30 postoperation, respectively.
Results
MMP-2 and -9 could not be detected immunohistochemically on the anterior capsulorhexis preoperation of cataract. Lens epithelia cells at the equator began to migrate by day 4. A confluent monolayer of lens epithelia cells was present on the posterior capsule at day 20. Total MMP-2 and -9 protein production increased with time with maximum levels reached on day 30.
Conclusion
MMP-2 and -9 were showed to be upregulated following sham cataract surgery. MMP expression could play an important role in PCO.
Similar content being viewed by others
Log in or create a free account to read this content
Gain free access to this article, as well as selected content from this journal and more on nature.com
or
References
Wong TT, Sethi C, Daniels JT . Matrix metalloproteinases in disease and repair processes in the anterior segment. Surv Ophthalmol 2002; 47: 239–256.
Ye HQ, Azar DT . Expression of gelatinase A and B and TIMPs 1 and 2 during corneal wound healing. Invest Ophthalmol Vis Sci 1998; 39: 913–921.
Mackay AR, Gomez DE, Cottam DW . Identification of the 72-kDa ( MMP-2 ) and 92-kDa ( MMP-9 ) gelatinase/type IV collagenase in preparations of laminin and Matrigel. Biotechniques 1993; 15: 1048–1051.
Liu CS, Wormstone IM, Duncan G, Marantonio JM, Webb SF, Davies PD . A study of human lens cell growth in vitro. A model for posterior capsule opacification. Invest Ophthalmol Vis Sci 1996; 37: 906–914.
Weinreb RN, Kashiwagi K, Kashiwagi F, Tsukahara S, Lindsey JD . Prostaglandins increase matrix mentalloproteinase release from human ciliary smooth muscle cells. Invest Ophthalmol Vis Sci 1997; 38: 2772–2780.
Tamiya S, Wormstone IM, Marcantonio JM, Gavrilovic J, Duncan G . Induction of matrix metallo proteinases 2 and 9 following stress to the lens. Exp Eye Res 2000; 71 (6): 591–597.
Wormstone IM, Tamiya S, Anderson I, Duncan G . TGF-β2-induced matrix modification and cell transdifferentiation in the human lens capsular bag. Invest Ophthalmol Vis Sci 2002; 43: 2301–2308.
Richiert DM, Ireland ME . Matrix metalloproteinase secretion is stimulated by TGF-b in cultured lens epithelial cells. Curr Eye Res 1999; 19: 269–275.
Wormstone IM, Del Rio-Tsonis K, McMahon G, Tamiya S, Davies P, Marcantonio J et al. FGF: an autocrine regulator of human lens cell growth independent of added stimuli. Invest Ophthalmol Vis Sci 2001; 42: 1305–1311.
Bermbach G, Mayer U, Naumann GO . Human lens epithelial cells in tissue culture. Exp Eye Res 1991; 52: 113–119.
Ohta K, Yamagami S, Taylor AW . IL-6 antagonizes TGF-beta and abolishes immune privilege in eyes with endotoxin-induced uveitis. Invest Ophthalmol Vis Sci 2000; 41: 2591–2599.
Acknowledgements
We thank Jing Liu of the Tongren Eye Bank for her invaluable work.
Author information
Authors and Affiliations
Corresponding author
Rights and permissions
About this article
Cite this article
Li, J., Wang, N. & Wang, J. Expression of matrix metalloproteinases of human lens epithelial cells in the cultured lens capsule bag. Eye 22, 439–444 (2008). https://doi.org/10.1038/sj.eye.6702735
Received:
Revised:
Accepted:
Published:
Issue date:
DOI: https://doi.org/10.1038/sj.eye.6702735
