Figure 1

Dot matrix analysis of the curLT and Tol1-tyr nucleotide sequences. curLT (abscissa) is a genomic DNA fragment of Oryzias curvinotus containing a 6.0-kb-long Tol1 copy. Tol1-tyr (ordinate) is a 1.9-kb-long Tol1 copy first found in O. latipes. The criterion for matching was a 70% match over a window of 20 nucleotides. The dot matrix shows that, in curLT, Tol1 is disrupted by an extra DNA fragment of 4.5 kb. Positions of possible stop codons are shown in the boxes under the dot matrix. The arrows in the boxes are ‘open reading frames’ of more than 150 amino acids included in the extra fragment. An ‘open reading frame’ was defined here as a stretch of amino acids not necessarily starting with the ATG codon, and is thus more loosely defined than this term’s usual meaning. The bars with letters a to d are regions used for probes in Southern hybridization experiments. Their locations on the EMBL AB081572 sequence are: a, 453-1252; b, 1353-2152; c, 2253-3052; d, 3153-4052.