Figure 5
From: Novel biomaterial strategies for controlled growth factor delivery for biomedical applications
Schematic of the preparation process of BMP-2 encapsulated chitosan/chondroitin sulfate NPs and immobilization of the NPs on porous biphasic calcium phosphate (BCP) scaffolds for the study of ectopic bone formation. (a) Preparation of BMP-2 encapsulated chitosan/chondroitin sulfate NPs (a), FITC labeled chitosan/chondroitin sulfate NPs uniformly distributed on the BCP scaffold, as revealed by a confocal laser scanning microscope (CLSM) (b), Immunohistochemical staining for collagen type I on deparaffinized sections of BMP-2 encapsulated NPs functionalized BCP scaffolds: extensive immunoreactive area (brown) is observed (c).
