Abstract
Aim:
To determine whether deguelin can regulate the expression of nuclear factor kappa B (NF-κB) binding protein (IκBα) in U937 human leukemia cells and Raji human B lymphoma cells.
Methods:
The localization of IκBα protein was investigated by using an immunofluorescence method. The expression of IκBα and NF-κB/p65 proteins in Raji and U937 cells were investigated by using Western blotting. Apoptosis was detected through annexin V/PI double-labeled cytometry.
Results:
IκBα localized in the cytoplasm in untreated and deguelin-treated cells. After treatment with tumor necrosis factor α (TNF-α) or deguelin plus TNF-α for 15 min, there was a substantial reduction in the amount of IκBα protein. The expression of IκBα was downregulated by deguelin in Raji and U937 cells. Deguelin induced apoptosis in U937 cells.
Conclusion:
Deguelin inhibited the expression of IκBα protein in U937 and Raji cells. The anti-proliferative activity of deguelin is related to the signal pathway of NF-κB.
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Project supported by the National Natural Science Foundation of China (No 30472267).
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Chen, Wh., Chen, Y. & Cui, Gh. Deguelin inhibits expression of IκBα protein in Raji and U937 cells. Acta Pharmacol Sin 27, 485–490 (2006). https://doi.org/10.1111/j.1745-7254.2006.00284.x
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DOI: https://doi.org/10.1111/j.1745-7254.2006.00284.x
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