Abstract
Aim:
To obtain pathophysiological meanings of lysophosphatidylcholine (LPC) through the investigation of the effects of LPC in Jurkat T cells.
Methods:
We measured ROS generation, [Ca2+]i, and mitochondrial membrane potential (MMP) by fluorescent spectrometry in Jurkat T cells.
Results:
We observed that LPC significantly increased the reactive oxygen species (ROS) level in human Jurkat T cells. Among structurally-related lysolipids and eleven synthetic LPCs with different acyl chain lengths, palmitoyl LPC increased ROS to the highest level. α-Tocopherol, an antioxidant, and rottlerin PKCδ inhibitor were inhibitory effects on LPC-induced ROS generation. LPC rapidly depolarized MMP and markedly elevated [Ca2+]i by Ca2+ influx across the plasma membrane. However, LPC-induced ROS increase seemed to not be related with LPC-induced depolarization of MMP or [Ca2+]i increase. G2A family G protein-coupled receptors (GPCR) for lysolipids were expressed in Jurkat T cells, however, evidence indicated that GPCR was not involved in LPC actions.
Conclusion:
LPC induced several cellular changes in Jurkat T cells, including an increase of ROS generation in a PKCδ-dependent and GPCR-independent manner, increase of [Ca2+]i through Ca2+ influx, and decrease of MMP. LPC-induced actions in Jurkat T cells represent novel action modes of LPC that do not involve GPCR and multiple independent changes of intracellular signaling molecules.
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Project supported by the Korea Research Foundation Grant (KRF-2004-041-E00061).
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Im, Yj., Lee, Yk., Chung, Hy. et al. Multiple actions of lysophosphatidylcholine in human Jurkat T cells. Acta Pharmacol Sin 27, 700–707 (2006). https://doi.org/10.1111/j.1745-7254.2006.00339.x
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DOI: https://doi.org/10.1111/j.1745-7254.2006.00339.x
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