Abstract
Aim:
To study the role of the tachykinin receptors in spontaneous contractions of longitudinal and circular smooth muscle from rabbit small intestine and to determine the mechanism of action of Substance P (SP).
Methods:
Rabbit duodenum, jejunum and ileum segments were prepared. The spontaneous contractions of longitudinal and circular smooth muscle were recorded using a computer via an isometric force transducer. The specific agonists and antagonists of tachykinin receptors were added into the organ bath.
Results:
The agonists of tachykinin NK1 receptor (SP and [Sar9] SP), NK2 receptor (NKA and (β-Ala8)-NKA), and NK3 receptor (NKB and Senktide) all induced contractions in the small intestine. The contractions were diminished by NK1 receptor antagonist L-733,060, NK2 receptor antagonist GR-94800, and NK3 receptor antagonist SB 218795. Contractions caused by SP were also reduced by atropine, verapamil, PKC inhibitor staurosporine, and PLC inhibitor U73122.
Conclusion:
Ttachykinin NK1, NK2, and NK3 receptors mediate the contractions of the smooth muscle in rabbit intestine. Furthermore, SP acts directly on smooth muscle cells through the tachykinin NK1 receptor.
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Acknowledgements
This work was supported by the Ministerio de Educación y Ciencia of Spain (AGL2006-04317 and ERDF) and the Grupo de Investigación Consolidado del Gobierno de Aragón (B61/2009, Spain).
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Valero, M., Fagundes, D., Grasa, L. et al. Contractile effect of tachykinins on rabbit small intestine. Acta Pharmacol Sin 32, 487–494 (2011). https://doi.org/10.1038/aps.2010.227
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DOI: https://doi.org/10.1038/aps.2010.227
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