Table 1 Determination of plitidepsin IC50 in human and murine JAK2V617F-mutated cell lines and controls

From: Evaluation of plitidepsin in patients with primary myelofibrosis and post polycythemia vera/essential thrombocythemia myelofibrosis: results of preclinical studies and a phase II clinical trial

Cell line

IC50 (n m)

 

WST-1 assay

Clonogenic assay

K562

1.50±0.10

2.70±0.30

HEL

1.00±0.30

1.50±0.05

SET2

1.00±0.30

0.80±0.02**

UKE-1

2.40±0.20

0.50±0.03**

Ba/F3 JAK2 wild-type

0.40±0.03

ND

Ba/F3 JAK2 V167F

0.03±0.01**

ND

  1. Abbreviations: IC50, plitidepsin concentration that reduced colony number to 50% that measured in control dishes with vehicle only; ND, not done.
  2. IC50 value was calculated using both short-term proliferation assay in liquid cultures and long-term clonogenic assay in agar. Control murine Ba/F3 wild-type cells were maintained in the presence of IL-3. **P<0.01.
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