Table 2 miRNAs and genes included in the network for which differential expression in PMF patients compared to healthy controls has been confirmed

From: A data-driven network model of primary myelofibrosis: transcriptional and post-transcriptional alterations in CD34+ cells

PMF vs CTR

CD34+

GR and Plasma

 

Arrays

RNA-seq

RT-PCR

RT-PCR (PMF vs CTR; RQ mean±dev)

miR-106a-5p

UP

  

UP GR (1.9±1.2 vs 1.08±1.0)

miR-145-5p

UP

  

UP GR (1.7±0.9 vs 1.0±0.5)

miR-146b-5p

UP

 

UP

 

miR-155-5p

UP

 

UP

 

miR-17-5p

UP

  

UP GR (1.5±0.7 vs 1.1±0.9)

miR-185-5p

UP

  

UP GR (3.0±3.1 vs 1.5±1.2)

miR-195-5p

UP

 

UP

UP GR (15.3±10.5 vs 1.8±2.1)

UP Plasma (12.4±17.5 vs 1.37±0.95)

miR-19a-3p

UP

 

UP

UP GR (15.3±10.5 vs 1.8±2.1)

UP Plasma (12.4±17.5 vs 1.37±0.95)

miR-19b-3p

UP

UP

UP

 

miR-21-5p

UP

 

UP

 

miR-29a-3p

UP

UP

UP

UP Plasma (3.0±3.1 vs 1.5±1.2)

miR-29c-3p

UP

 

UP

 

miR-494-3p

UP

UP

UP

 

ANGPT1

UP

 

UP

 

ARHGEF7

DOWN

 

DOWN

 

CDC42

DOWN

 

DOWN

 

MEF2D

DOWN

 

DOWN

 

SMAD7

DOWN

 

DOWN

 
  1. Abbreviations: miRNA, microRNA; PMF, primary myelofibrosis.
  2. The second column indicates if miRNAs and genes resulted up- or down-regulated according to array data used for network reconstruction. The following columns indicate for which elements a significant differential expression has been previously confirmed using RNA-seq in CD34+ cells,22 and using RT-PCR in CD34+ cells,20 in granulocytes (GR) and in plasma samples.
Back to article page