Figure 3

Proteosome-mediated degradation of Mcl-1 and Bcl-2 in HMLE cells can be attenuated by proteosome inhibitor MG132. (A) CHX-treated cells were treated with or without MG132 and subjected to western blot analysis using anti-Mcl-1 and anti-Bcl-2 antibodies. (B) CHX-treated cells were treated with or without MG132 and subjected to fluorometric assay for caspase-3 activity. Data represent mean±s.e.m. of three independent experiments done in quadruplicates (the asterisks denote statistical significant values compared with CHX+MG132 treatment groups, ^**P⩽0.01. (C) CHX-treated cells were treated with or without MG132 and subjected to western blot analysis using anti-Ub antibody.