Figure 2

Concordance of TP63 probeset with p63 protein immunohistochemistry. (A) SCC (V562, V558 and V554) and AC (V505, V524 and V594) tumours, stained for p63 by immunohistochemistry ( × 5). Brown indicates p63 and blue indicates haematoxylin nuclear counterstain. (B) High magnification ( × 20) example of V592 and V594 demonstrating the specificity of the nuclear stain. (C) Cluster dendrogram of the 19 TP63 probesets detected above background (DABG P<0.01) in our SCC and AC samples. Clustered by samples and by probesets. The scaled expression of each probeset, denoted as the row Z-score, is plotted in red–blue colour scale with red indicating high expression and blue indicating low expression. LIMMA P-value for each probeset is displayed in brackets. p63 IHC result for each sample is indicated by the p63 bar, coloured black for positive and white for negative.