Figure 2
From: FADD protein release mirrors the development and aggressiveness of human non-small cell lung cancer
Fas-associated death domain protein is released by human NSCLC. The amount of FADD released by T (black) and by distant NT (white) biopsies in vitro was determined by ELISA. (A) Fas-associated death domain protein release by different samples (diamond) of the same biopsy is shown for seven patients. Bars represent the mean value for each patient. Histogram represents the mean s.d. of FADD release for T and NT patients. (B) Fas-associated death domain protein release by T and NT biopsies from 50 NSCLC patients. When several samples of a same biopsy were tested, we used the samples' mean value. Each diamond represents a patient. Bars represent the mean value for the entire cohort. P values were determined using the Wilcoxon signed-rank test. Range for T and NT biopsies were from 5.3 to 339.7 and 1.2 to 122.5 ng mg−1 PT, respectively. (C) Spearman’s correlation. (D and E) Fas-associated death domain protein release depends on the NSCLC histological type. Levels of released FADD determined in Figure 2B were reanalysed after the 50 NSCLC patients were classified according to their histological type. Each diamond represents a patient biopsy (T in black, NT in white). Bars represent the mean value (D). Histograms represent the mean released FADD value ±s.e.m. P values were determined using the Mann–Whitney test (E).
