Figure 1 | British Journal of Cancer

Figure 1

From: Hsa-mir-124-3 CpG island methylation is associated with advanced tumours and disease recurrence of patients with clear cell renal cell carcinoma

Figure 1

(A) Structure of the mir-124-3 CGI and location of the qMSP assay relative to the mir-124-3 transcription start site (TSS). Vertical lines represent CpG sites within the CGI. Chromosomal positions refer to the GRCh37/hg19 annotation in the UCSC genome browser (Lander et al, 2001; Kent et al, 2002). (B) Exemplary primary data of quantitative methylation-specific and control PCR measurements in duplicate for A498 (1); methylated control DNA (2); unmethylated control DNA (3); unconverted DNA (4) and a blank control (5). (C) Normalised mir-124-3 assay threshold values (Ct) for a two-fold dilution series of the methylated control in non-methylated control DNA for determination of assay linearity and efficiency.

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