Figure 5
SW IV-52s and SW III-123 induced TNF α -dependent apoptosis. (A) SKOV-3 cells were pre-treated with or without 2 μg ml–1 TNFα antibody for 1 h, and then treated with 3 μ M SW IV-52s, SW 43 or SW III-123 for 24 h. The cells were assayed for caspase 3 activity. (B–D) SKOV-3 cells were pre-treated with or without 2 μg ml–1 TNFα antibody for 1 h, and then treated with 3 or 10 μ M SW 43 (B), SW IV-52s (C) or SW III-123 (D) for 48 h. Viability of cells was determined by MTS assay. The representative data from three independent experiments are shown. *P<0.05.
