Figure 1
From: IFN-γ from lymphocytes induces PD-L1 expression and promotes progression of ovarian cancer
PD-L1 expression on ovarian cancer cells is induced by IFN- γ. (A) PD-L1 expression in cell lines from the CCLE data set. The horizontal line at value 4.950718 represents the determined cutoff value, as mentioned in the Materials and Methods section. (B) Expression of IFNGR1 and IFNGR2 in cell lines of ovarian origin from the CCLE data set. The horizontal and vertical lines represent the determined cutoff values. (C) PD-L1 expression in ascites tumour cells from four cases of ovarian cancer. CD326-positive and 7-AAD-negative cells were gated as viable tumour cells. Open histogram with grey line: anti-PD-L1 antibody; filled histogram: isotype control. PD-L1-positive cases (lower panels) and -negative cases (upper panels) are shown. (D) Ascites cells designated by * in (C) were incubated with (upper panel) or without (lower panel) IFN-γ for 24 h, and PD-L1 expression is shown. Open histogram with grey line: anti-PD-L1 antibody; filled histogram: isotype control.
