Figure 2
From: Anti-apoptotic role and clinical relevance of neurotrophins in diffuse large B-cell lymphomas
Comparative study of the pattern expression of neurotrophins and their receptors in GCB- and ABC-like DLBCL cell lines. (A) Analysis of NGF, BDNF, NT3, and their receptor expression in cell lysates of two GCB-like (SUDHL4 and SUDHL6) and two ABC-like (OCI-LY3 and OCI-LY10) cell lines, by western blotting after a 72-h culture. Blots were re-probed with anti-β-actin as a loading control. Data are representative of at least four independent experiments. (B) Immunofluorescence staining performed without cell permeabilisation in DLBCL cell lines confirmed surface Trk receptors and neurotrophins or p75NTR expression on tumour B cells (revealed by Alexa Fluor 488, in green or Alexa Fluor 594, in red). Merged image (yellow) revealed NTs/Trk or Trk/ p75NTR co-localisation in DLBCL (in blue: DAPI staining). Data are done for SUDHL6 to compare with OCI-LY10 and are representative of at least three independent experiments. (C) TrkB-phosphotyrosine Y817 level was revealed by western blotting in all DLBCL cell lysates after a 72 h culture in basal conditions. (D) Protein lysates from all cell lines were subjected to immunoprecipitation (IP) with anti-p75NTR or anti-phospho TrkB (Y817) followed by immunoblotting (western blotting) with indicated antibodies, showing p75NTR interaction with Trk receptors but not with sortilin. Data are representative of three independent experiments. The full colour version of this figure is available at British Journal of Cancer online.
