Figure 1

The IKK α deletion in the intestinal epithelium reduces adenoma formation and proliferation. (A) Schematic representation of the strategy used to generate the compound mice used in our study. (B) Representative images of H&E staining of Apc^Min/+;Ikkα^+/+ and Apc^Min/+;Ikkα^−/− intestinal Swiss rolls. Dashed lines delimited two different tumours present in the Apc^Min/+;Ikkα^+/+ image. (C) Quantification of tumour number in the Apc^Min/+;IKKα^+/+ and APC^Min/+;IKKα^−/− intestinal Swiss rolls. (D) Representative images of Ki67 immunohistochemistry (IHC) in Apc^Min/+; Ikkα^+/+ and Ikkα^−/− adenomas. (E) Quantification of Ki67-positive cells shown in (D). (F) Immunohistochemistry of Ki67 in Ikkα^+/+ and Ikkα^−/− intestines and (G) quantification of the number of Ki67-positive cells per hemi-crypt. Graphs represent the average number of tumours (C) or percentage of cells (E and G) per Swiss roll from animals of each genotype. (H and I) The IHC analysis of β-catenin (H) and quantification of percentage of tumour cells of each genotype showing nuclear β-catenin staining (I). (J and K) The IHC of cleaved caspase 3 in tumours of the indicated genotypes (J) or obtained from mice irradiated 3 h before processing the samples (K). Representative images for (H, J and K) are shown. For statistical analysis, ordinary one-way ANOVA or unpaired t-test was used and the P-values are indicated as ***P<0.001 and ****P<0.0001.