Figure 2
From: IKKα is required in the intestinal epithelial cells for tumour stemness
Ikkα−/− tumouroids show a decrease in size and proliferation. (A) Representative images of growing tumouroids from the indicated genotypes. Western blot analysis of IKKα is shown in the right panel. P=passage. (B) Quantification of tumouroid length from three independent experiments performed. (C) Representative images of organoid cultures from nontransformed Ikkα+/+ and Ikkα−/− intestinal stem cells and western blot analysis to demonstrate the efficiency of Ikkα deletion. Western blot showing the levels of Ikkα in a representative culture from the indicated genotype (right panel). (D) Immunofluorescence (IF) staining of Ki67 in the ApcMin/+ Ikkα+/+ and Ikkα−/− tumouroids from passage 6 grown in Matrigel (Corning). (E) Quantification of the percentage of Ki67-positive cells from three independent experiments that was performed as in (D). The graph shows the average percentage and s.d. of the mean. Magnification of images is indicated. For statistical analysis we used ordinary one-way ANOVA or unpaired t-test. P-values are indicated as *P<0.05, ***P<0.001 and ****P<0.0001.
